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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,276)
Mouse Secondary Antibodies (14,583)
Rabbit Secondary Antibodies (6,323)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,242)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (77)
Horse Secondary Antibodies (75)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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3,256 – 3,270 of 72,202 results
3,256

Goat anti-Rat IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rat
Host Species Goat
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry (Paraffin)
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Goat anti-Rat IgG (H+L) Secondary Antibody was developed against rat IgG.
Primary or Secondary Secondary
3,257

Goat anti-Mouse IgG2c Heavy Chain Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Goat
Conjugate DyLight 594
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Antigen IgG2c Heavy Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Mouse IgG2c
Primary or Secondary Secondary
3,258

Goat anti-Feline IgG (H+L) Secondary Antibody, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Antigen IgG (H+L)
Content And Storage Store at 4°C.
Purification Method Affinity purified
Conjugate Unconjugated
Target Species Feline
Host Species Goat
Applications Flow Cytometry,Immunomicroscopy
Formulation 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.05% Sodium Azide
Classification Polyclonal
Immunogen Purified Cat IgG (H&L)
Isotype IgG
Primary or Secondary Secondary
3,259

Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, Rhodamine (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Guinea Pig
Host Species Goat
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with No Preservative
Classification Polyclonal
Immunogen Guinea Pig IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
3,260

Goat anti-Equine IgM Heavy Chain Secondary Antibody, DyLight 550, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Equine
Host Species Goat
Conjugate DyLight 550
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline B Cell Development and Differentiation Markers, Immunology
Antigen IgM Heavy Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Gene Alias Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene ID (Entrez) 3507
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Horse IgM
Primary or Secondary Secondary
3,261

Goat anti-Rat IgG2a Heavy Chain Secondary Antibody, Novus Biologicals™
SDP

Goat Polyclonal Antibody has been used in 5 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Rat
Host Species Goat
Conjugate Unconjugated
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Concentration 1.0 mg/mL
Antigen IgG2a Heavy Chain
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000-1:30000, ELISA 1:1000-1:30000 (Primary) 1:100-1:500 (for coating plates), Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2000, Immunohistochemistry-Paraffin 1:200 - 1:2000
Classification Polyclonal
Immunogen Rat IgG2a
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with Rat IgG2a. This may cross react with IgG2a from other species.
3,262
Promotions Available

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody, R&D Systems™
GREENER_CHOICE SDP

Goat Polyclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Mouse
Host Species Goat
Conjugate PerCP
Applications Flow Cytometry
Isotype IgG
Antigen IgG
Gene Symbols IGHG1
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 10 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Immunogen Mouse IgG heavy and light chains
Classification Polyclonal
Primary or Secondary Secondary
Test Specificity Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab') 2 fragments which have reduced interactions with cell surface Fc receptors
3,263

Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, DyLight 633, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Guinea Pig
Host Species Goat
Conjugate DyLight 633
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Guinea Pig IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on guinea pig IgG and light chains on all guinea pig immunoglobulins
3,264

Goat anti-Human IgG (H+L) Secondary Antibody, DyLight 680 (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Human
Host Species Goat
Conjugate DyLight 680
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Human IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Human IgG and with light chains of most Human immunoglobulins.
3,265

Goat anti-Human Lambda Light Chain Secondary Antibody, PE, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Goat
Conjugate PE
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen Lambda Light Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Gene Alias IGLC, immunoglobulin lambda constant group
Gene ID (Entrez) 3537
Formulation PBS
Classification Polyclonal
Immunogen Human Lambda Light Chain
Primary or Secondary Secondary
3,266

Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Mouse
Host Species Goat
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Antigen IgA/IgG/IgM (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000
Classification Polyclonal
Immunogen Mouse IgG IgA and IgM whole molecule
Primary or Secondary Secondary
Test Specificity This reagent is suitable for the detection of all Mouse immunoglobulin isotypes, subclasses and chain combinations.
3,267

Goat anti-Rabbit IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Goat
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunoprecipitation
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Goat anti-Rabbit IgG (H+L) Secondary Antibody was produced by repeated immunization with rabbit whole IgG molecule in goat.
Primary or Secondary Secondary
3,268

Goat anti-Canine IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Canine
Host Species Goat
Conjugate Janelia Fluor 669
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunomicroscopy
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Goat anti-Canine IgG (H+L) Secondary Antibody was developed against purified canine IgG (H&L).
Primary or Secondary Secondary
3,269

Goat anti-Hamster IgG (H+L) Secondary Antibody, DyLight 680 (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Hamster
Host Species Goat
Conjugate DyLight 680
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:10000
Classification Polyclonal
Immunogen Armenian and Golden Syrian Hamster IgG, whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Mouse and Rat Serum Proteins. No reaction was observed against Mouse or Rat Serum Proteins. This antibody will react with heavy chains of Golden Syrian & Armenian Hamster IgG and with light chains of most Golden Syrian & Armenian Hamster immunoglobulins.
3,270

Goat anti-Mouse IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Goat
Conjugate PerCP
Applications ChIP Assay,Dot Blot,ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation PBS
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary